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Rock pocket mouse worksheet answers5/30/2023 ![]() ![]() The mouse GFAP probe cross-hybridizes weakly at high stringency with genomic DNA from diverse eukaryotic species. a.What specific trait did researchers study in this investigation Fur color, specifically melanism, is the trait they studied. As you watch, record the following information. Southern blot analysis indicates the existence of at most two genes encoding GFAP in the mouse genome. The activities focus on the MC1R gene, protein, and receptor, to teach valuable lessons on mutation, selection, and convergent evolution, and to tie together biochemistry, cell communication, and. MAKING OF THE FITTEST: ROCK POCKET MOUSE ANSWER SHEET PROCEDURE 1.Watch the short film The Making of the Fittest: Natural Selection and Adaptation. under similar conditions, natural selection can favor similar. different mutations in two different genes cannot generate the same phenotype. dark fur color evolved independently on each lava flow. They then use a genetic code chart, to determine the amino acids encoded by a sequence of the Mc1r gene in different mouse populations. If your class covers the Hardy-Weinberg equilibrium, you may wish to use the other film lesson, Allele and Phenotype Frequencies in Rock Pocket Mouse. The activity begins with students reading a magazine article and watching the film. ![]() ![]() there are at least two genes involved in creating dark-colored mouse fur. Students analyze genetic sequence data and draw conclusions about the evolution of coat-color phenotypes in the rock pocket mouse. RNA blot transfer experiments using the cloned GFAP cDNA probe revealed a single GFAP mRNA species of 2.7 kilobases in mouse brain. dark-colored fr evolved only once in rock pocket mice. Within the coding region, the data show extensive homology with other intermediate filament proteins, particularly in those regions predicted to be alpha-helical. in the rock pocket mouse populations at location B. The clone includes a long (1.4-kilobase) 3& amp #39 untranslated region. Occurred due to a random mutation and was not favoured because the mouse would. The complete sequence of the cDNA insert in this clone is presented, encompassing 2.5 kilobases and specifying greater than 97% of the GFAP amino acid sequence. The library was screened using a GFAP-specific polyclonal antiserum a single bacterial colony expressing GFAP was identified. A clone encoding mouse glial fibrillary acidic protein (GFAP) was isolated from a cDNA library constructed so as to express the cloned sequences. ![]()
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